Automated chemical and genetic in vivo screening of zebrafish
The Centre for Discovery Brain Sciences at the University of Edinburgh hosts the UK Zebrafish Screening Facility.
At the centre of the facility is an automated zebrafish embryo handling system (VAST, Union Biometrica), which combined with a high speed spinning disk confocal microscope (Zeiss/Yokogawa) allows for rapid zebrafish screening.
The facility also benefits from a Zeiss LSM880 microscope with Airyscan, allowing for super resolution imaging in vivo. Together these two microscopes allow for rapid in vivo chemical screening, with follow up at the sub-cellular level.
To further increase the throughput and robustness of our screening assays, we have fully automated image processing and analysis for several of our assays, with more to come.
The UK Zebrafish Screening Facility is open to academic or industry collaborators from the UK and internationally.