What do we do Project designOptical clearing (RatDISCO, iDISCO and CLARITY)Whole brain Light-sheet microscopy imaging Consultation on image analysis (FIJI, QUINT, Arivis) RatDISCO We have developed a tissue processing protocol to efficiently and effectively optically clear and immunolabel whole rat brains: RatDISCO (Edinburgh Innovation disclosure application EI0000698). Contact us Please get in touch with us for a detailed quotation:cristina.martinez-gonzalez@ed.ac.ukkirsty.craigie@ed.ac.uk Dr Cristina Martínez González Reseach Fellow and Facility Manager Contact details Email: cristina.martinez-gonzalez@ed.ac.uk Web: Website Kirsty Craigie Senior Technical Officer Contact details Email: kirsty.craigie@ed.ac.uk Useful links Dodt, H.-U. et al. Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain. Nat. Methods 4, 331–336 (2007).Ye, L. et al. Wiring and Molecular Features of Prefrontal Ensembles Representing Distinct Experiences. Cell 165, 1776–1788 (2016).Renier, N. et al. IDISCO: A simple, rapid method to immunolabel large tissue samples for volume imaging. Cell 159, 896–910 (2014).Ueda, H. R. et al. Tissue clearing and its applications in neuroscience. Nat. Rev. Neurosci. 21, 61–79 (2020). This article was published on 2024-09-09
