Microscopes

The UltraMicroscope Blaze (Miltenyi Biotech) is a light sheet microscope designed for high-throughput 3D imaging of large/multiple samples. 

The system has 5 lasers and 3 objectives (1.1X, 4X and 12X with added magnification) providing a total magnification from 0.6 - 30X and a working distance from 10 - 17mm.

A standard sample chamber accommodates multiple rodent organs/organoids, while the XXL upgrade expands the capacity for human kidneys or a whole adult mouse. 

The Ultramicroscope II (Miltenyi Biotech) is a light-sheet microscope with a single-objective capable of 1.2x up to 12.6x magnification (Zoom body configuration) and a working distance of 6mm.

The system can image samples such as a mouse or a rat brain (FOV lateral: 1.7–17.6 mm), and it is compatible with water-based and solvent-based optical clearing.

The Zeiss Axio Scan Z1 slide scanner:

• Holds up to 100 normal glass slides.
• It can image both bright-field and up to 4-colour fluorescent samples (405, 488, 556 and 633 nm excitation).
• Regions of interest can be automatically detected or manually marked on multiple samples on each slide.
• Setup wizard can guide users through optimising image acquisition and autofocus parameters.
• Once setup, the system can be left for unattended automated scanning e.g. overnight.
• It is equipped with a state-of-the-art Hamamatsu ORCA-Flash camera and 5x, 10x and 20X objectives.